• Non-contact ultrasonic
  • LAWSON20-A
  • Sample processing quantities: 30 x 0.1 mL, 20 x 0.65 mL, 11 x 2 mL, and 5 centrifuge tubes of 5-15 mL.
  • LAWSON20-A
  • Non-contact ultrasonic
  • Sample processing quantities: 30 x 0.1 mL, 20 x 0.65 mL, 11 x 2 mL, and 5 centrifuge tubes of 5-15 mL.
LAWSON20-A Non-contact ultrasonic uses isothermal and non-contact methods to disrupt, homogenize, and mix samples. It is used for sterile, ultra-low-volume crushing and can break chromosomes through centrifuge tubes.
Product Introduction
LAWSON20-A Non-contact ultrasonic  isothermal and non-contact methods to disrupt, homogenize, and mix samples. It is used for sterile, ultra-low-volume crushing and can break chromosomes through centrifuge tubes. It is specially tailored for pre-processing of second-generation sequencing DNA samples and chromatin immunoprecipitation experimental samples. Compared to traditional probe ultrasonic homogenizers, which directly contact the probe with the sample and can only process one sample at a time, resulting in a long experimental cycle. When dealing with thousands of samples, it is necessary to reuse the same probe, which can easily cause sample cross-contamination. The non-contact sample can be crushed in a closed container without producing infectious aerosols. The ultrasonic probe does not contact the sample, avoiding cross-contamination. For laboratories that process multiple samples per day or handle valuable samples, non-contact ultrasonic DNA homogenizers have the advantages of high throughput, low sample loss, and no cross-contamination. It has gradually become an indispensable standardized tool for ChlP (chromatin immunoprecipitation) and DNA cutting research platforms.


Working principle
The non-contact ultrasonic gene disruptor uses a design that installs the ultrasonic generator at the bottom of the water tank. In traditional probe ultrasonic disruptors, the microflow phenomenon caused by ultrasonic waves can only occur in the area close to the probe. However, the non-contact ultrasonic cell pulverizer installs the ultrasonic generator at the bottom of the water tank, making the entire tank within the range of ultrasonic waves. The distribution of ultrasonic action is wide and balanced, reducing the formation of foam. During the experiment, the non-contact ultrasonic cell pulverizer's automatic and continuous rotation of centrifuge tubes ensures that the ultrasonic energy is distributed more evenly. During the experiment, the samples are placed in separate fully sealed centrifuge tubes, and there is no cross-contamination between the samples, avoiding the spread of aerosols.


Advantages
Traditional probe-type ultrasonic cell disruptors have direct contact between the probe and the sample, leading to metal ion contamination and long experimental cycles, as only one sample can be processed at a time. For multiple samples, the same probe must be reused, which can easily cause sample cross-contamination. Additionally, because the depth of the probe insertion and the energy distribution of each ultrasound are not consistent, the repeatability and accuracy of experimental results are affected. Furthermore, because a closed system cannot be used, the aerosols or foam produced during the ultrasonic process can spread into the environment, posing potential biological hazards. In contrast, the non-contact ultrasonic cell disruptor can detect 4-32 samples simultaneously, with high experimental efficiency. The probe does not require frequent operation, and each sample is in a separate fully enclosed test tube, avoiding cross-contamination. The use of a 4-degree water bath ensures a uniform distribution of ultrasonic energy and full ultrasonic action. The ultrasonic parameters can be flexibly set, and the experimental steps are standardized, resulting in good experimental repeatability and high reliability of the results.


Product Features
⒈ No aerosol float produced - enhanced biological safety (e.g. branch bacteria, viruses, etc.)
⒉ Eliminates the risk of sample cross-contamination
⒊ Eliminates the traditional problem of probe wear and debris
⒋ Can process a wide range of samples with a broad sample processing range
⒌ Suitable for various standard containers
⒍ Can be used for processing trace samples, as small as 5ul
⒎ The automatic continuous rotation of the centrifuge tube makes the energy distribution of the ultrasonic wave more uniform
⒏ Optional high and low temperature constant temperature water bath, customized rotating base for Eppendorf tubes of various diameters according to customer needs
Advantages
non-contact ultrasonic processors
【1】High throughput, up to 60 samples can be processed simultaneously, with high experimental efficiency.
【2】No frequent operation of the probe is required, and each sample is in a separate fully enclosed test tube to avoid cross-contamination.
【3】The product is equipped with a variety of adapters ranging from 0.1ml to 15ml, suitable for different types of samples.
【4】The ultrasonic parameters can be flexibly set, and the experimental steps are standardized, with good experimental reproducibility and high result reliability.
【5】No special consumables are required, resulting in low experimental costs.
【6】The use of ultrasonic waves in a 4°C low-temperature water bath allows for even energy distribution and complete ultrasonic effects, with samples processed fully in a low-temperature environment to prevent denaturation.


Experimental Results
Experimental purpose: Fragmentation of collected cell DNA samples: 4T1 cells derived from mouse mammary gland cancer.
Instrument: Non-contact ultrasonic DNA fragmentation instrument.
non-contact ultrasonic processors


Experimental data presentation of ultrasonic gene disruptor
Experimental objective:
1. Test the disruption time of different fragment sizes under the breaking condition of 50ul, 100% power (300W), ultrasonic time: 10s, interval time: 10s.
2. Verify the repeatability and uniformity of disruption effect under the same disruption condition.
Test the effect of ultrasonic disruption damage on the yield of the product recovery.
non-contact ultrasonic processors
non-contact ultrasonic processors


Experimental Conclusion
1. For a 50 ul reaction system (in 0.2 ml thin-wall PCR tube), recommended sonication time for DNA fragments with sizes of 400-600 bp is 2 min; for fragments with sizes of 300 bp, it is 4 min; and for probe capture, it is recommended to sonicate for 7-10 min.
2. With the increase of sonication time, the size of sonicated fragments becomes smaller with certain regularity.
3. Under the same sonication conditions, the reproducibility and uniformity of the sonication effect are good.
4. The longer the sonication time, the smaller the sonicated fragments, and the lower the DNA gel recovery rate.


Application Range
1. Fragmentation of DNA samples for second-generation sequencing.
2. Fragmentation of RNA.
3. Disruption of bacteria and cells.
4. ChIP assay (chromatin immunoprecipitation).
5. Sample preparation for high-throughput sequencing instruments.
6. Extraction of membrane proteins and other proteins.
7. Homogenization and emulsification reactions.
8. Ultrasonic treatment of expensive reagents.
9. Immunoprecipitation experiments and catalytic reactions.


Technical Specifications    
Model: LAWSON20-A single-channel LAWSON30-A dual-channel
Power: 10-1000W continuously adjustable 25-2500W continuously adjustable
Sample processing quantity: 30*0.1ml, 20*0.65ml, 11*2ml and 5 5-15ml centrifuge tubes 60*0.1ml, 40*0.65ml, 22*2ml and 10 5-15ml centrifuge tubes
Sonication sample volume: 2ml or more, or less than 5µL 2ml or more, or less than 5µL
Single sonication time: 0.1-99.9S 0.1-99.9S
Single interval time: 0.1-99.9S 0.1-99.9S
Total time (sonication+interval): 1-99H59M59S 1-99H59M59S
Frequency range: 20-40KHz 20-40KHz
Cooling system - temperature control range: Portably constant temperature bath host (compressor cooling) 2~40℃ Portably constant temperature bath host (compressor cooling) 2~40℃
Temperature reading accuracy: ±0.1 ℃ ±0.1 ℃
Compressor power: 600W 600W
Ultrasonic tank volume: 15*14*10cm 23*14*10cm
Adapter material: 316 stainless steel material 316 stainless steel material
Noise level: <55dB <55dB
Imported original multi-frequency ultrasonic transducer: 3 sets 6 sets
Power supply (optional 110V): 220V/110V/ 50Hz/60HZ 220V/110V/ 50Hz/60HZ

 

Product List
LAWSON20-A Unit
Host Machine Unit
Special Cooling Equipment Set
Adapters set
Power cord piece
User manual copy
Warranty card copy
Certificate of qualification copy
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